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双岐杆菌是革兰氏阳性、不产芽孢的厌氧菌,主要存在于人和一些动物的肠道中,具有阻止有害细菌滋生和感染的作用。双岐杆菌能选择性水解低聚果糖,而哺乳动物的消化酶不能水解低聚果糖。 微生物中的β—呋喃果糖苷酶可分为两类,一类为蔗糖酶,它能水解蔗糖但不水解菊糖;另一类为外菊糖酶(β-果糖苷酶),它不仅水解蔗糖也能水解菊糖,它们对蔗糖和菊糖的活性比相差甚大。有些青春双岐杆菌提取物水解1-蔗果三糖的速度比蔗糖和菊糖快,并能从1-蔗果三糖产生果糖和蔗糖。青春双岐杆菌G-1菌株的提取物对1-蔗果三糖的活性很高,当底物浓度各为5mM时,它对1-蔗果三
Bifidobacterium is a gram-positive, non-spore-forming anaerobic bacteria, mainly in humans and some animals in the intestine, with the role of preventing harmful bacteria and infection. Bifidobacteria selectively hydrolyze fructooligosaccharides, whereas mammalian digestive enzymes do not hydrolyze fructo-oligosaccharides. Β-fructofuranosidase in microorganisms can be divided into two categories, one is sucrase, which hydrolyzes sucrose but does not hydrolyze inulin; the other is exo-inulinase (β-fructosidase) which not only hydrolyzes Sucrose also hydrolyzes inulin, which varies greatly in activity ratio of sucrose and inulin. Some B. bifidum extracts hydrolyze 1-cacosan trisaccharide faster than sucrose and inulin, and can produce fructose and sucrose from 1-cacao trisaccharide. The extract of Bifidobacterium adolescentis strain G-1 has a high activity on 1-caccatane tacrose, and when the substrate concentration is 5 mM each, the activity of 1-