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分别从对氰烯菌酯敏感和抗性禾谷镰刀菌菌株中诱导了22个和50个nit突变体。通过比较它们的生物学特性表明,nit突变体在菌丝生长速率、培养形状以及致病性方面与其亲本没有显著差异,但是无性繁殖和有性繁殖能力有所改变。同时实验还表明,禾谷镰刀菌对氰烯菌酯和氯酸盐不存在交互抗性,且对氰烯菌酯和氯酸盐的双重抗性能够稳定地遗传。因此,可以将nit作为一个优良的遗传标记研究禾谷镰刀菌对氰烯菌酯的抗药性遗传。另外成功运用nit作为分子标记研究了禾谷镰刀菌对氰烯菌酯的抗药性在菌丝融合过程中的遗传和变异。研究结果表明,抗药性基因不能通过菌丝融合传递给另一个菌株或发生的概率极低,这将不利于对氰烯菌酯的抗性群体的发展。因此,菌丝融合在禾谷镰孢菌对氰烯菌酯的抗药性群体发展中的作用较小。
Twenty-two and fifty nit mutants were induced from the Fusarium graminearum-resistant and susceptible strains, respectively. By comparing their biological characteristics, nit mutants showed no significant differences in their mycelial growth rate, culture shape and pathogenicity, but their vegetative and sexual reproductive abilities changed. At the same time, the experiment also showed that Fusarium graminearum had no cross-resistance to the mycotoxins and chlorates, and the double resistance to the cyanogenic ester and chlorate could be stably inherited. Therefore, nitric as an excellent genetic marker could be used to study the genetic inheritance of resistance to gramycin in Fusarium graminearum. In addition, nitrite was successfully used as a molecular marker to study the inheritance and variation of Fusarium graminearum resistance to the mycotoxins in mycelial fusion. The results show that the resistance gene can not be transmitted to another strain through mycelial fusion or the probability of occurrence is very low, which will not be conducive to the development of resistance groups to the bacterial strain. Therefore, mycelial fusion has less effect on the development of drug resistant colonies of Fusarium graminearum against the bacterial strain of cystatin.