论文部分内容阅读
以小麦(Triticum aestivum L.)为试验材料,采用完全营养液进行培养,外加适当浓度的过氧化氢(H2O2),同时以不加H2O2为对照,再以不同浓度Pb溶液培养进行Pb胁迫,检测小麦幼苗过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)含量、根系活力及微核率等指标的变化,以探究外源H2O2对Pb胁迫下小麦抗氧化酶及其细胞活性的影响。结果表明,Pb胁迫下小麦幼苗的生长代谢受到一定程度的抑制,主要表现为根系活力下降,POD、SOD、CAT活性增强,MDA含量和微核率上升;经适当浓度H2O2处理后,Pb胁迫小麦幼苗中POD、SOD、CAT活性及根系活力与对照相比有不同程度地上升,MDA含量及微核率下降,这表明外加H2O2对Pb胁迫小麦所受的伤害有一定的缓解作用,可增强小麦幼苗对重金属Pb的抗性。
Wheat (Triticum aestivum L.) was used as the experimental material and completely nutrient solution was used for cultivation. Hydrogen peroxide (H2O2) was added at the appropriate concentration. At the same time, with no H2O2 as the control, Pb stress was detected by different concentrations of Pb solution. The activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) Effects of Exogenous H2O2 on Antioxidase and Cell Activity of Wheat under Pb2 + Stress. The results showed that under Pb stress, the growth and metabolism of wheat seedlings were inhibited to a certain extent, the root activity decreased, the activities of POD, SOD and CAT increased, while the content of MDA and micronucleus increased. After treated with appropriate concentration of H2O2, Compared with the control, POD, SOD, CAT activity and root vigor of seedlings increased to some extent, MDA content and micronucleus rate decreased, which indicated that addition of H2O2 could alleviate the damage of Pb-stressed wheat and enhance wheat Seedling resistance to heavy metal Pb.