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水稻基因组DNA用PstⅠ酶切同时与人工接头连接后,使用选择性引物进行PCR扩增,琼脂糖凝胶电泳检测所构建的水稻DNA指纹图谱。结果表明在JX17和ZYQ8间以及5种野生稻间均存在DNA多态性片段。
After the rice genomic DNA was digested with PstI and ligated with the artificial linker, the PCR products were amplified by selective primers. The DNA fingerprinting of rice was detected by agarose gel electrophoresis. The results showed that there were DNA polymorphic fragments between JX17 and ZYQ8 and five wild rice species.