目的探讨fascin1基因表达对胃癌细胞系MKN45增殖与凋亡的影响及其意义。方法利用RNA interference(RNAi)技术稳定沉默fascin1基因的表达,Western blotting及RT-PCR检测干扰效率,用MTT比色法和平板克隆形成实验检测转染前后细胞体外增殖能力,用流式细胞术(FCM)及Ho-chest33258荧光染色法检测细胞凋亡的变化。结果在稳定转染fascin1干扰质粒后,胃癌细胞系MKN45的fascin1表达明显下降;与未转染组和空白质粒转染组相比,fascin1干扰质粒转染组的增殖能力明显降低,但细胞凋亡变化不明显。结论fascin1的表达与胃癌细胞系MKN45的增殖有关,而与凋亡无明显相关。 Objective To investigate the effect and significance of fascin1 gene expression on proliferation and apoptosis of gastric cancer cell line MKN45. Methods The expression of fascin 1 gene was silenced by RNA interference (RNAi). The interference efficiency was detected by Western blotting and RT-PCR. The proliferation of cells was detected by MTT assay and plate clone formation assay. Flow cytometry FCM) and Ho-chest33258 fluorescence staining were used to detect the changes of apoptosis. Results The expression of fascin 1 in gastric cancer cell line MKN45 was significantly decreased after stable transfection of fascin 1 plasmid. Compared with untransfected group and blank plasmid transfected group, the proliferation of fascin 1 transfection group was significantly decreased, but the apoptosis Change is not obvious. Conclusion The expression of fascin1 is related to the proliferation of gastric cancer cell line MKN45, but not to apoptosis.