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目的了解秦皇岛市海产品中血蚶、牡蛎、海虹受诺如病毒污染情况,检测3种贝类带毒率,对检测出的诺如病毒进行GⅠ和GⅡ分型。建立Mengo病毒作为阳性质控检测病毒回收率方法。方法蛋白酶水解法富集病毒,全自动核酸提取仪提取病毒RNA,实时荧光定量PCR方法对诺如病毒进行检测和分型,用Mengo病毒做标准曲线,检测诺如病毒回收率。结果 Mengo病毒回收率均>1%符合富集要求,检测牡蛎、海虹、血蚶各20份,检出诺如病毒阳性样本11份,其中GⅠ型1株,GⅡ型10株,总检出率为18.33%。牡蛎、血蚶均检出GⅡ型毒株,检出率分别为30.00%、5.00%,海虹检出GⅠ型和GⅡ型毒株,检出率分别为5.00%和15.00%。结论 3种贝类产品均受到诺如病毒不同程度的污染,GⅡ毒株含量较多,牡蚶和海虹受到污染较为严重。
Objective To understand the pollution of Norovirus in blood, oysters and Haihong in the seafoods of Qinhuangdao, detect the virulence rates of three species of shellfish and genotype GⅠ and GⅡ detected in Norovirus. Establishment of Mengo virus as a positive control for detection of virus recovery. Methods The virus was enriched by protease hydrolysis, the RNA was extracted by automatic nucleic acid extractor and Norovirus was detected by real - time fluorescence quantitative PCR. The standard curve of Meno virus was used to detect the recovery of Norovirus. Results The recoveries of Mengo virus were all higher than 1%. According to the enrichment requirements, 20 samples of oyster, sea rainbow and blood bank were detected respectively. Eleven specimens of Norovirus were detected, including 1 strain of GⅠ type and 10 strains of G Ⅱ type. The rate was 18.33%. Oyster, blood 蚶 were detected G Ⅱ strains, the detection rates were 30.00%, 5.00%, Hai Hong detected G Ⅰ and G Ⅱ strains, the detection rates were 5.00% and 15.00%. Conclusions The three kinds of shellfish products are all contaminated with norovirus, and the content of GⅡ strain is more, and the pollution of mullet and sea rainbow is more serious.