Spectrum sensing is an essential component to realize the cognitive radio, and the requirement for real-time spectrum sensing in the case of lacking prior information, fading channel, and noise uncertainty, indeed poses a major challenge to the classical spectrum sensing algorithms. Based on the stochastic properties of scalar transformation of power spectral density(PSD), a novel spectrum sensing algorithm, referred to as the power spectral density split cancellation method(PSC), is proposed in this paper. The PSC makes use of a scalar value as a test statistic, which is the ratio of each subband power to the full band power. Besides, by exploiting the asymptotic normality and independence of Fourier transform,the distribution of the ratio and the mathematical expressions for the probabilities of false alarm and detection in different channel models are derived. Further, the exact closed-form expression of decision threshold is calculated in accordance with Neyman–Pearson criterion. Analytical and simulation results show that the PSC is invulnerable to noise uncertainty,and can achive excellent detection performance without prior knowledge in additive white Gaussian noise and flat slow fading channels. In addition, the PSC benefits from a low computational cost, which can be completed in microseconds. Spectrum sensing is an essential component to realize the cognitive radio, and the requirement for real-time spectrum sensing in the case of lacking prior information, fading channel, and noise uncertainty, indeed poses a major challenge to the classical spectrum sensing algorithms. Based on the stochastic properties of scalar transformation of power spectral density (PSD), a novel spectrum sensing algorithm, referred to as the power spectral density split cancellation method (PSC), is proposed in this paper. The PSC makes use of a scalar value as a test, the ratio of each subband power to the full band power. Besides, by exploiting the asymptotic normality and independence of Fourier transform, the distribution of the ratio and the mathematical expressions for the probabilities of false alarm and detection in different channel Models are derived. Further, the exact closed-form expression of decision threshold is calculated in accordance with Neyman-Pearson criterion. Analytical and simulation results show that the PSC is invulnerable to noise uncertainty, and can achive excellent detection performance without prior knowledge in additive white Gaussian noise and flat slow fading channels. In addition, the PSC benefits from a low computational cost, which can be completed in microseconds.