采用高效液相色谱 /质谱法 ,对蘑菇柄中 5′ 核苷酸进行了分析。在C18柱上 ,以含 0 0 5%磷酸的水 甲醇 ( 95/5,V/V)为流动相进行了分离 ,检测波长 2 60nm。以HPLC与LC/MS复合定性蘑菇柄中5′ 核苷酸为 5′ 鸟苷酸、5′ 尿苷酸、5′ 腺苷酸和 5′ 胞苷酸。采用外标法定量 ,它们的回收率分别为98 9%、95 1 %、94 1 %和 1 0 1 4 % ,检测限分别为 2 8、2 .7、3.5和 4 .3mg/L。 The 5 ’nucleotides in the mushroom stems were analyzed by high performance liquid chromatography / mass spectrometry. On a C18 column, the separation was carried out with methanol-water (95/5, V / V) containing 0.05% phosphoric acid as the mobile phase at a wavelength of 260 nm. The 5 ’nucleotides in the mushroom stems were 5’ guanylic acid, 5 ’uridylic acid, 5’ adenylic acid and 5 ’cytidine acid by HPLC and LC / MS. Using external standard method, their recoveries were 98 9%, 95 1%, 94 1% and 1 0 1 4%, respectively. The detection limits were 28.2, 3.7, 3.5 and 4.3 mg / L, respectively.