目的:测定氯地滴眼液中氯霉素和地塞米松磷酸钠的含量。方法:采用高效液相色谱法,ODS柱,流动相为甲醇-0.34％磷酸二氢钾(60:40),流速1.0 ml·min-1,检测波长240 nm。结果:氯霉素和地塞米松磷酸钠的平均回收率为99.8％,RSD=0.4％和99.5％,RSD=0.5％。结论:方法快速、准确,可作为该制剂的质量控制方法。 Objective: To determine the content of chloramphenicol and dexamethasone sodium phosphate in chlorhexidine eye drops. Methods: ODS column was used. The mobile phase consisted of methanol-0.34% potassium dihydrogen phosphate (60:40), the flow rate was 1.0 ml · min-1 and the detection wavelength was 240 nm. Results: The average recoveries of chloramphenicol and dexamethasone sodium phosphate were 99.8%, RSD = 0.4% and 99.5%, RSD = 0.5%. Conclusion: The method is rapid and accurate and can be used as a quality control method for this preparation.