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目的建立不同产地藤茶的HPLC指纹图谱与一测多评方法,为藤茶的质量控制提供实验依据。方法采用Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.5%甲酸水溶液进行梯度洗脱,检测波长为258、292、369 nm,柱温为50℃,体积流量为0.8 m L/min,并应用SPSS 19.0软件对所得数据进行聚类分析和主成分分析,对不同产地藤茶样品进行质量分类。结果建立了不同产地藤茶的HPLC指纹图谱,确定了24个共有峰,鉴定了3个主要共有峰,分别为二氢杨梅素、杨梅素和杨梅苷,并建立了对不同产地藤茶中二氢杨梅素、杨梅素和杨梅苷的一测多评方法。结论建立的结合指纹图谱、模式识别和一测多评法的质量控制模式可应用于藤茶的质量评价。
OBJECTIVE: To establish HPLC fingerprinting and multi-evaluation methods of rattan tea from different habitats to provide experimental evidence for the quality control of rattan tea. Methods The mobile phase consisted of methanol-0.5% formic acid aqueous solution with gradient elution at Agilent Eclipse Plus C18 (250 mm × 4.6 mm, 5 μm). The detection wavelength was 258, 292, 369 nm and the column temperature was 50 ℃. 0.8 m L / min. SPSS 19.0 software was used to carry out cluster analysis and principal component analysis on the obtained data to classify the cane tea samples from different areas. Results HPLC fingerprint of rattan tea from different habitats was established and 24 common peaks were identified. Three major common peaks were identified, including dihydromyricetin, myricetin and myricitrin. Hydrogen Myricetin, myricetin and myricitin a test evaluation method. Conclusion The established quality control mode combining fingerprinting, pattern recognition and multi-assessment can be applied to the quality evaluation of rattan tea.