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目的:研究放射复合伤口愈合中外周血 T 淋巴细胞亚群的变化规律及其与愈合延迟的关系。方法:用碱磷酶免疫组织化学方法检测 T 淋巴细胞亚群。结果:伤口区病理形态学观察表明,大剂量局部照射后能导致伤口愈合延迟;照射后白细胞数出现下降,伤后3～5d 降低较为明显,显著低于单纯伤口组;伤后两组动物外周血 T 淋巴细胞及其亚群均出现降低,然而照射组始终低于单伤组,至伤后28d 仍然有显著性差别。结论:照射后外周血 T 淋巴细胞及其亚群的降低和白细胞数的下降是辐射延迟伤口愈合的重要原因
OBJECTIVE: To investigate the changes of T lymphocyte subsets in peripheral blood and the relationship with delayed healing in radiotherapy wound healing. Methods: T lymphocyte subsets were detected by alkaline phosphatase immunohistochemistry. Results: The morphological observation of the wound area showed that the wound healing was delayed after high-dose local irradiation. The number of leukocytes decreased after irradiation, and the decrease was obvious at 3 ~ 5 d after wounding, which was significantly lower than that of the simple wound group. Blood T lymphocytes and its subgroups all decreased, however, the irradiation group was always lower than the single injury group, and there was still significant difference between the 28th day after injury. Conclusion: The decrease of peripheral blood T lymphocytes and their subpopulations and the decrease of white blood cell count after radiation are the important reasons for radiation delayed wound healing