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目的:探讨人类微小病毒B19感染(parvovirusB19,B19)与甲状腺乳头状癌(Papillarythyroidcarcinoma,PTC)的关系。方法:对38例手术切除的PTC患者甲状腺组织(其中30例伴有周围正常甲状腺组织)及16例甲状腺腺瘤患者腺瘤旁正常甲状腺组织,分别用巢式聚合酶链反应(nestedpolymerasechainreaction,nPCR)、原位杂交(insituhybridization,ISH)和免疫组织化学(immunohistochemistry,IHC)检测B19病毒DNA和病毒蛋白的表达。结果:nPCR扩增出173bp的B19特异性目的条带。ISH和IHC检测出B19病毒DNA和蛋白分别位于肿瘤细胞胞核和胞浆内。在PTC中,nPCR、ISH和IHC阳性率分别为97.4%(37/38)、78.9%(30/38)和63.2%(24/38),而腺瘤旁正常甲状腺组织中,阳性率分别为43.8%(7/16)、12.5%(2/16)和6.25%(1/16)。两者比较差异有统计学意义(3种方法均P<0.001)。癌旁甲状腺组织ISH和IHC阳性率分别为23.3%(7/30)和10.0%(3/30),与之相对应的30例PTC(ISH:80.0%;IHC:60.0%)相比,差异有统计学意义(两者均P<0.001)。结论:PTC患者甲状腺肿瘤组织中B19感染率明显高于腺瘤旁和癌旁正常甲状腺组织,提示B19感染在PTC的发生中可能起重要的作用。
Objective: To investigate the relationship between parvovirus B19 and B19 and papillary thyroid carcinoma (PTC). Methods Thirty-eight thyroidectomized thyroid tissues (including 30 normal thyroid tissues) and 16 normal thyroid adenoma tissues were retrospectively analyzed by nested polymerase chain reaction (nPCR) , In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of B19 viral DNA and viral proteins. Results: nBPCR amplified 173 bp B19-specific target band. ISH and IHC detected B19 virus DNA and protein were located in the tumor cell nucleus and cytoplasm. The positive rates of nPCR, ISH and IHC in PTC were 97.4% (37/38), 78.9% (30/38) and 63.2% (24/38), respectively. The positive rates of nPCR, ISH and IHC in normal thyroid tissue adjacent to adenoma were 43.8% (7/16), 12.5% (2/16) and 6.25% (1/16). The difference was statistically significant (P <0.001 for all three methods). The positive rates of ISH and IHC in paraneoplastic thyroid tissue were 23.3% (7/30) and 10.0% (3/30) respectively, which were significantly different from the corresponding 30 cases of PTC (ISH: 80.0%; IHC: 60.0%) Statistically significant (P <0.001 for both). Conclusion: The infection rate of B19 in thyroid tumor tissues of patients with PTC is obviously higher than that of normal thyroid tissues adjacent to adenoma and para-carcinoma, suggesting that B19 infection may play an important role in the pathogenesis of PTC.