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应用厌氧毒性试验方法评价了五氯酚对厌氧颗粒污泥利用葡萄糖、挥发性脂肪酸（ＶＦＡ）及产甲烷毒性的影响。结果表明，厌氧颗粒污泥利用葡萄糖、ＶＦＡ的活性及产甲烷活性均随ＰＣＰ浓度的增加而明显下降。其中产甲烷活性更易受ＰＣＰ的抑制；反应器中、下层颗粒污泥比上层及未驯化颗粒污泥对ＰＣＰ的抑制有较高的忍耐性，且其活性恢复也较快；颗粒污泥中乙酸产甲烷菌和互营丙酸盐降解菌比互营丁酸盐降解菌对ＰＣＰ更敏感。
The anaerobic toxicity test was used to evaluate the effects of pentachlorophenol on the utilization of glucose, volatile fatty acids (VFA) and methanogenic toxicity of anaerobic granular sludge. The results showed that the anaerobic granular sludge utilization of glucose, VFA activity and methanogenic activity were significantly decreased with the increase of PCP concentration. The methanogenic activity was more easily inhibited by PCP. In the reactor, the lower granular sludge had higher tolerance to PCP inhibition than the upper and untreated granular sludge, and the activity recovery was also faster. In the granular sludge, acetic acid Methanogens and their associated propionate-degrading bacteria are more sensitive to PCP than the obligate butyrate-degrading bacteria.