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The viaB gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transfomed into Salmonella typhimurium x4072, an attenuated △ cya △ crp mutant. Recombinant S.typhimurium Vi4072 had the ability t0 produce Vi capsular polysaccharide and also to invade and colonize the small intestine, mesenteric lymph nodes, and spleen of BALB/c mice. Mice orally immunized with. Vi4072 developed serum and secretory antibody responses to the Vi antigen, as measured by a passiVe
The viaB gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was obtained from pMY2 and transfomed into Salmonella typhimurium x4072, an attenuated Delta cya delta crp mutant. Recombinant S. typhimurium Vi4072 had the ability t0 produce Vi capsular polysaccharide and also to invade and colonize the small intestine, mesenteric lymph nodes, and spleen of BALB / c mice. Mice orally immunized with. Vi4072 developed serum and secretory antibody responses to the Vi antigen, as measured by a passiVe