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目的 建立人脂肪细胞载脂蛋白E基因表达检测方法。方法 采用竞争性逆转录 -聚合酶链式反应 (RT -PCR)方法分析儿童脂肪细胞的载脂蛋白E基因表达。结果 载脂蛋白E基因能在儿童脂肪细胞表达 ,儿童载脂蛋白E基因表达量为( 0 41± 0 12 )。结论 采用竞争性逆转录 -聚合酶链式反应方法检测人脂肪细胞载脂蛋白E基因表达方法快速、简便、灵敏、实用、可靠 ,且能准确定量 ,值得推荐
Objective To establish a method for the detection of apolipoprotein E gene expression in human adipocytes. Methods The apolipoprotein E gene expression in pediatric adipocytes was analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR). Results The apolipoprotein E gene was expressed in childhood adipocytes. The expression of apolipoprotein E gene in children was (0 41 ± 0 12). Conclusion The method of competitive reverse transcription polymerase chain reaction (RT-PCR) to detect the expression of apolipoprotein E in human adipocytes is rapid, simple, sensitive, practical, reliable and accurate. It is recommended