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AIM:To explore the activation of signal transduction pathwaysrelated with the carcinogenesis of sporadic colon cancers.METHODS:A gene array monitoring the activation of 8 signaltransduction pathways(PathwayFinder GEArray)was usedto screen the differentially expressed genes betweencolorectal cancer and normal colon tissues.The differentiallyexpressed genes were further analyzed by RT-PCR,usingRNA derived from colorectal cancer and normal colon tissueof 35 patients.RESULTS:The expression of HSF1,HSF27,HSP90 and iNOSwas increased in colon cancer tissues compared to normalcolon tissue using PathwayFinder GEArray.The RT-PCRresults showed that the expression of HSF1 was increasedin 86%(30/35)patients and the expression of iNOS wasincreased in 63%(22/35)patients.CONCLUSION:The induction of HSF1 gene expression isassociated with sporadic colon cancer.HSF1 induces heatshock stress signaling pathway,which might play a role inthe carcinogenesis of sporadic colorectal cancer.Cen H,Zheng S,Fang YM,Tang XP,Dong Q.Induction ofHSF1 expression is associated with sporadic colorectal cancer.World J Gastroenterol 2004;10(21):3122-3126http://www.wjgnet.com/1007-9327/10/3122.asp
AIM: To explore the activation of signal transduction pathways associated with the carcinogenesis of sporadic colon cancers. METHODS: A gene array monitoring the activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes betweencolorectal cancer and normal colon tissues. The differentiallyexpressed genes were further analyzed by RT-PCR, using RNA derived from colorectal cancer and normal colon tissue of 35 patients. RESULTS: The expression of HSF1, HSF27, HSP90 and iNOS was increased in colon cancer tissues compared to normal colone tissue using Pathway GEA. The RT-PCR results showed that the expression of HSF1 was increasedin 86% (30/35) patients and the expression of iNOS was established in 63% (22/35) patients.CONCLUSION: The induction of HSF1 gene expression isassociated with sporadic colon cancer. HSF1 induces heatshock stress signaling pathway, which might play a role in carcinogenesis of sporadic colorectal cancer. Chen H, Zheng S, Fang YM, Tang XP, Dong Q. Induction of HSF1 expression is associated with sporadic colorectal cancer. World J Gastroenterol 2004; 10 (21): 3122-3126http: //www.wjgnet.com/1007-9327/10/3122.asp