Studying the metallogenic age of Dapinggou gold deposit may provide important material not only for the study of metallization and the further prospecting of gold deposits but also for research into tectonic evolution in the Altun area. In this study, we used Rb-Sr isotopic dating method of fluid inclusion in the Au-bearing quartz, combining field investigation, REE, sulfur isotopes, lead isotopes, strontium isotopes and hydrogen-oxygen isotope, to determine the metallogenic age of the Dapinggou gold deposit. The only newly-discovered medium-sized gold deposit in the northern Altun area, Dapinggou gold deposit, is controlled by a ductile shear zone. It is characterized by mainly altered mylonite-types with a little K-feldspar quartz vein-types. Data from REE, sulfur isotopes, lead isotopes and strontium isotopes show that the source materials of the deposit were derived mainly from the metamorphic rocks. The data from the inclusion and hydrogen-oxygen isotope indicate that the ore-forming fluid came mainly from dynamic metamorphic water and partly from remelting magmatic water, with a little influence from meteoric water. The density of the ore-forming fluid is 0.791-0.971 g/cm3 and it contains 2.36 %-5.5 % salinity. The gold deposit was formed at 1.61-2.68 km below the surface at a temperature of 198-290 ℃ and a pressure of (420-700)×105 Pa. The isotopic age from the Rb-Sr isochron of fluid inclusion in quartz is 487 Ma, which indicates that the mineralization occurred in the early Caledonian epoch. The study on the metallogenic age of Dapinggou gold deposit may provide important material not only for the study of metallization and the further prospecting of gold deposits but also for research into tectonic evolution in the Altun area.