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亚欧唐松草细胞悬浮培养物的次生代谢物——小蘖碱是一种药物.本文叙述了各种植物生长激素(2,4-D,NAA,IAA,IBA)和细胞激动素(6-苄基腺嘌呤,BAP)的不同浓度对亚欧唐松草悬浮培养细胞的生长及小蘖碱产量的影响,揭示了亚欧唐松草悬浮培养细胞TDW株系的最佳生长条件及使小蘖碱产量最高的激素条件,从而提出两段培养的方法.主要实验如下:(1)在同样条件下,测定不同生长素分别对细胞生长及小蘖碱产量的影响.在不加入细胞激动素时,培养基中低浓度2,4-D(1μM)促进细
The berberine, a secondary metabolite of the cell suspension culture of T. arjuna, is a drug. This article describes various plant growth hormones (2,4-D, NAA, IAA, IBA) and cytokinins (6 Effects of Different Concentrations of Benzyl Adenine and BAP) on the Growth and Berberine Production of Thalassiosia tsutsugamushi Suspension Culture Cells Alkaline production yields the highest hormonal conditions, thus suggesting a two-stage culture method. The main experiments are as follows: (1) Under the same conditions, the effect of different auxin on cell growth and berberine yield was measured. When no cytokinin was added Medium, low concentration of 2,4-D (1μM) promotes fine