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六十年代以来用植物组织培养的方法,诱导产生了许多植物的类胚体。但是对于山茶科油茶(Camellia oleifera abel)体细胞胚状体的发生尚未见报道。广西林科所生理研究室1978年以来对此进行了实验研究,并获得成功。据试验,油茶体细胞胚状体发生在山茶种子的子叶基部,在培养过程中,不经愈伤组织阶段,直接从表皮细胞分化出吊挂型胚状体。试验中,他们对油茶体细胞胚状体的发生进行了连续切片观察,结果说明:发生在油茶子叶基部的胚状体发育是不同步的,在同一个外植体上可以看到球形胚、心形胚和子叶形胚。油茶体细胞胚状体是从子叶基部分化而来
Since the 1960s, plant tissue culture has been used to induce the production of many plant embryoid bodies. However, the occurrence of somatic embryoid bodies of Camellia oleifera abel has not been reported yet. Guangxi Linke Institute of Physiology has carried out experimental research since 1978, and achieved success. According to the experiment, Camellia somatic embryogenesis occurs at the base of the cotyledons of camellia seeds. During the culture process, the embryoid bodies of the hanging type are differentiated directly from the epidermal cells without the callus stage. In the experiment, they observed the occurrence of somatic embryogenesis in Camellia oleifera. The results showed that the development of embryoid bodies occurred at the base of Camellia oleifera was asynchronous, the globular embryos could be seen on the same explant, Heart-shaped and cotyledons. Camellia somatic embryoid body is derived from the cotyledon base