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甘蓝型油菜是一种重要的油料作物,为了改良其种子脂肪酸组分,提升其经济价值,本研究分析了油菜种子发育时期脂肪酸合成积累模式及BnFAD2、BnFAD3、BnFATB基因的表达规律,认为这3个基因在种子发育中后期(授粉后25d起)的高效表达对油酸合成积累有着重要影响。通过Napin启动子诱导对油菜植株中BnFAD2、BnFAD3、BnFATB基因进行RNAi共干扰抑制,以达到提升油酸含量的目的。试验结果表明,转基因油菜种子中BnFAD2、BnFAD3、BnFATB基因的表达受到强烈抑制,种子中油酸含量由66.76%提升至82.98%,且油脂合成的相关基因同步出现表达上调。
In order to improve its seed fatty acid composition and enhance its economic value, this study analyzed the accumulation pattern of fatty acid synthesis and the expression regularity of BnFAD2, BnFAD3 and BnFATB during the seed development stage of rapeseed (Brassica napus L.) The high expression of these genes in the late seed development stage (25d after pollination) has an important influence on the accumulation of oleic acid. The Napin promoter was used to induce RNAi co-suppression of BnFAD2, BnFAD3 and BnFATB genes in rapeseed so as to enhance the content of oleic acid. The results showed that the expression of BnFAD2, BnFAD3 and BnFATB in the seeds of transgenic rapeseed was strongly inhibited, the content of oleic acid in seeds increased from 66.76% to 82.98%, and the genes related to the synthesis of oil were up-regulated simultaneously.