近年来组合生物学技术在开发抗生素类似物的研究中发挥着越来越重要的作用,在他克莫司类似物的组合生物合成中,通过应用外源合成前体取代烯丙基丙二酸单酰基和4,5-二羟基-1-烯-环己甲酸,以及应用模块化合成元件取代AM合成元件和改造后修饰合成元件,已经生产出11个他克莫司类似物,这些研究拓宽了人们对标准化合成前体和模块化合成元件的认识,为通过组合生物合成手段进行抗生素的更新换代提供指导。 In recent years, combinatorial biology has played an increasingly important role in the development of antibiotic analogues. In the combinatorial biosynthesis of tacrolimus analogs, allylmalonic acid Monoacyl and 4,5-dihydroxy-1-ene-cyclohexanecarboxylic acid, as well as the use of modular synthetic elements to replace AM synthetic elements and modified synthetic elements have been produced 11 tacrolimus analogues, these studies broaden People’s understanding of the standardization of synthetic precursors and modular synthesis of components, for the combination of biosynthetic means to provide guidance for the replacement of antibiotics.