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菌核病是油菜的主要病害之一,目前尚未在油菜及相关植物中找到抗性基因。油菜等植物中的PGIP蛋白对核盘菌的致病因子PG具有诱导性防卫反应作用,有关PGIP蛋白的防卫反应机制研究已成为热点。本研究利用同源序列法,从甘蓝型油菜宁RS-1中克隆得到1个尚未报道的PGIP基因家族新成员——PGIP18。通过生物信息学分析发现,该基因的DNA具有1段长97bp的内含子序列和两段外显子序列,编码的氨基酸序列与甘蓝型油菜PGIP2亚家族蛋白同源性较高,且都由保守的N端信号肽和富含亮氨酸的LRR重复结构域组成。该基因的克隆为揭示油菜中内源PGIP基因的抗病机制和功能研究提供了新的基因资源。
Sclerotinia is one of the major diseases of rapeseed. At present, no resistance gene has been found in rape and related plants. PGIP protein in plants such as rapeseed has an inductive defense response to pathogenicity factor PG of Sclerotinia, and research on the defense response mechanism of PGIP protein has become a hot topic. In this study, a new member of the PGIP gene family, PGIP18, was cloned from Brassica napus RS-1 using homologous sequence method. Bioinformatics analysis revealed that the DNA of this gene has a 97-bp intron sequence and two exon sequences. The deduced amino acid sequence has high homology with the PGIP2 subfamily protein of Brassica napus, A conserved N-terminal signal peptide and a leucine-rich LRR repeat domain. The cloning of this gene provides new gene resources for revealing the mechanism and function of the endogenous PGIP gene in rapeseed.