论文部分内容阅读
对分离自玉米植株的内生细菌R-4进行鉴定,测试其对玉米南方锈病(SCR)的防病增产效果。用盆栽方法测定菌株R-4对玉米植株的促生效果,根据16S r DNA序列对菌株R-4进行鉴定,采用自然发病方式测试其对SCR的防病增产效果。结果表明,菌株R-4处理玉米的发芽率、播种后18 d株高、播种后28 d株高、植株地上部干重及根干重比对照分别增加17.19%、32.03%、41.80%、92.53%和37.03%。通过16S r DNA序列分析,将菌株R-4鉴定为蜡样芽孢杆菌(Bacillus cereus)。菌株R-4在先玉335、银糯1号、浚单20上对SCR的防效达39.2%~92.1%(平均为65.7%),经该菌处理后玉米增产2.58%~9.02%(平均为6.66%)。
The endophytic bacteria R-4 isolated from maize plants were identified and their disease-controlling and yield-increasing effects on Southern Corn rust (SCR) were tested. Pot experiment was conducted to determine the promoting effect of strain R-4 on maize plants. The strain R-4 was identified according to the 16S r DNA sequence. The disease-resistant and yield-increasing effects on SCR were tested by natural methods. The results showed that the germination rate of maize R-4 was 17.19%, 32.03%, 41.80% and 92.53% higher than that of the control at the 18th day after sowing, % And 37.03%. Strain R-4 was identified as Bacillus cereus by 16S r DNA sequence analysis. The control efficiency of SCR-R-4 strain was 39.2% ~ 92.1% (averagely 65.7%) in Xian-yu 335, Yinuoyu-1 and Xundan 20 and increased by 2.58% -9.02% 6.66%).