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目的建立金黄色葡萄球菌(金葡菌)的分子分型技术,研究深圳市食物中毒来源金葡菌的分子流行病学特征。方法对食物中毒事件中食物链的不同环节均采集标本,进行金葡菌分离培养。对所分离菌株同时应用SPA分型及PFGE技术作分子流行病学分析。结果 136起疑似食物中毒事件中有12起能够从食物链不同环节分离到金葡菌,共59株。PFGE将59株金葡菌分为15个型别,在10起食物中毒事件的不同环节中分析到PFGE型别一致的菌株,分辨系数为0.87。SPA分型将59株金葡菌分为10个型别,在12起食物中毒事件中不同环节样品中分析到SPA型别一致的菌株,分辨系数为0.85。结论 PFGE的分辨力高于SPA分型;通过结合流行病学资料分析,12起食物中毒事件中有6起能够通过分子分型方法准确溯源。
Objective To establish a molecular typing technique of Staphylococcus aureus (Staphylococcus aureus) to study the molecular epidemiology of Staphylococcus aureus in food poisoning in Shenzhen. Methods Samples were collected from different stages of food poisoning in food poisoning and were isolated and cultured in Staphylococcus aureus. The isolated strains were simultaneously applied SPA typing and PFGE molecular epidemiological analysis. Results Of 136 suspected food poisoning incidents, 12 were able to separate Staphylococcus aureus from 59 different stages of the food chain. 59 strains of Staphylococcus aureus were divided into 15 types by PFGE. PFGE-type strains were analyzed in different links of 10 food poisoning incidents with a resolution coefficient of 0.87. SPA typing divided 59 Staphylococcus aureus strains into 10 types. Among the 12 food poisoning cases, strains with the same SPA type were analyzed in different links with a resolution coefficient of 0.85. Conclusion The resolution of PFGE is higher than that of SPA. According to the epidemiological analysis, 6 of 12 food poisoning cases can be accurately traced by molecular typing.