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为定位分析石蜡包埋肝组织中微量HCVRNA,改良建立了原位PCR检测技术,并对17例丙型肝炎相关的肝细胞癌患者癌组织和癌周肝组织石蜡包埋切片进行检测。结果显示:该技术的特异性可靠,敏感性高于原位杂交法;两种组织切片HCVRNA阳性率分别为41.2%和70.6%,阳性信号主要呈胞浆型分布;在癌细胞中核型和核膜型分布比例较癌周肝组织明显增高,提示HCV可能与宿主细胞基因存在着相互作用。
In order to locate and analyze trace HCVRNA in paraffin-embedded liver tissue, an in situ PCR technique was established and 17 paraffin-embedded sections of 17 patients with hepatocellular carcinoma associated with hepatitis C and para-carcinoma liver tissues were detected. The results showed that the specificity and reliability of this technique were higher than that of in situ hybridization. The positive rates of HCV RNA in the two tissue sections were 41.2% and 70.6%, respectively, and the positive signals were mainly cytoplasmic. The distribution of nuclear type and nuclear membrane type was significantly higher than that of peri-cancerous liver tissue, suggesting that HCV may interact with host cell genes.