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目的:研究扇贝多肽(PCF)对中波紫外线(UVB)辐射人真皮成纤维细胞线粒体的影响。方法:检测丙二醛(MDA)含量以及细胞内抗氧化酶(SOD、GSH-PX)的活性;流式细胞术测定线粒体膜电位;透射电镜观察细胞超微结构的变化。结果:UVB(1.176×10~(-4)J·cm~(-2))导致真皮成纤维细胞线粒体损伤,PCF(0.25％-1％)剂量依赖性地减轻UVB对线粒体的损伤;而且,PCF也可剂量依赖性地维持线粒体膜电位的相对稳定,PCF能够减少MDA的生成量,提高SOD及GSH-PX的活性,PCF各组与UVB模型组相比差异有显著性(P<0.05,P<0.01)。结论:PCF保护成纤维细胞的线粒体免受UVB的损伤。
Objective: To study the effect of scallop polypeptide (PCF) on the mitochondria of human dermal fibroblasts irradiated by ultraviolet-ray (UVB). Methods: The content of malondialdehyde (MDA) and the activity of intracellular antioxidant enzyme (SOD, GSH-PX) were measured. The mitochondrial membrane potential was measured by flow cytometry. The ultrastructure of cells was observed by transmission electron microscopy. Results: UVB (1.176 × 10 -4 J · cm -2) resulted in mitochondrial damage in dermal fibroblasts. PCF (0.25% -1%) dose-dependently reduced mitochondrial damage induced by UVB. Moreover, PCF could maintain the relative stability of mitochondrial membrane potential in a dose-dependent manner. PCF could reduce the production of MDA and increase the activity of SOD and GSH-PX. There was significant difference between PCF group and UVB model group (P <0.05, P <0.01). Conclusion: PCF protects mitochondria of fibroblasts from UVB damage.