论文部分内容阅读
目的:研究稀土杂多化合物(PTW-6)体外抗乙型肝炎病毒(HBV)的活性。方法:MTT法检测PTW-6对Hep G2 2.2.15细胞的毒性,乙型肝炎病毒e(s)抗原诊断试剂盒检测上清液中HBeAg、HBsAg的含量,Southern blotting法检测PTW-6对细胞内HBV DNA复制的抑制作用。荧光定量PCR分析PTW-6对细胞内HBV mRNA和上清液中HBV DNA含量的影响。结果:PTW-6对2.2.15细胞的半数中毒浓度为1590.46 mg.L-1,PTW-6各浓度实验组对HBeAg和HBsAg的抑制率均高于对照组(P<0.05);随着PTW-6浓度的增高,PTW-6对2.2.15细胞内外HBV DNA的抑制率增加,PTW-6对2.2.15细胞外HBV DNA和细胞内HBV mRNA半数抑制浓度分别为51.1和63.6 mg.L-1。结论:PTW-6体外毒性较低,且对HBV复制有较好的抑制作用。
Objective: To study the anti-hepatitis B virus (HBV) activity of rare earth heteropoly compounds (PTW-6) in vitro. Methods: The toxicity of PTW-6 on Hep G2 2.2.15 cells was detected by MTT assay. The contents of HBeAg and HBsAg in supernatant were detected by Hepatitis B virus e (s) antigen kit. Inhibition of HBV DNA replication in vivo. Fluorescent quantitative PCR analysis of PTW-6 on intracellular HBV mRNA and supernatant HBV DNA content. Results: The half-poisoning concentration of PTW-6 in 2.2.15 cells was 1590.46 mg.L-1. The inhibition rates of HBeAg and HBsAg in PTW-6 groups were all higher than those in control group (P <0.05) -6, PTW-6 increased the inhibitory rate of HBV DNA in 2.2.15 cells, the half inhibitory concentration of PTW-6 on 2.2.15 extracellular HBV DNA and intracellular HBV mRNA were 51.1 and 63.6 mg.L- 1. Conclusion: PTW-6 is less toxic in vitro and has good inhibitory effect on HBV replication.