在血液学领域中,用过氧化物酶染色法来鉴别白血病的病型,其临床的重要性至今没有改变。用联苯胺作为本染色的基质,虽操作简便、结果稳定,但近年来发现生产联苯胺工人有发生膀胱癌的,并已明确联苯胺是致癌因素。1973年以来联苯胺的生产、贩卖、使用受到极大限制。因此,迫切需要改变此种方法。稻垣等开创了用芴衍生物作基质的过氧化物酶染色法,代替了联苯胺。用芴衍生物染色的原理 In the field of hematology, the clinical significance of the use of peroxidase staining for the identification of leukemias has not changed so far. Benzidine as the staining of the matrix, although the operation is simple, the results are stable, but in recent years found that the production of benzidine workers have bladder cancer, and benzidine has been clearly carcinogenic factors. Since 1973 benzidine production, sale, use has been greatly restricted. Therefore, there is an urgent need to change this method. Inagaki pioneered the use of fluorene derivatives as a substrate for peroxidase staining, instead of benzidine. The principle of dyeing with fluorene derivatives