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目的探讨非特异性T细胞疫苗(TCV)对空肠弯曲菌诱导的系统性红斑狼疮(SLE)样综合征小鼠免疫功能的影响。方法采用甲醛化空肠弯曲菌CF-1株辅以佐剂诱导近交系BALB/c小鼠SLE样自身免疫综合征病理模型,然后用非特异性TCV对该病理模型进行治疗。用酶联免疫吸附试验(ELISA)法检测小鼠血清抗dsDNA抗体、抗空肠弯曲菌CF-1外膜蛋白(CF-OMP)抗体水平,并检测特异性迟发型超敏反应(DTH),用苏木素-伊红(HE)染色检测肾脏病损。结果①非特异性TCV一方面使模型鼠抗dsDNA抗体水平升高,另一方面却使血清抗CF-OMP抗体水平一定程度的降低;②非特异性TCV明显抑制CF-1模型鼠对空肠弯曲菌特异性的DTH反应;③非特异性TCV能部分减轻模型鼠的肾脏病损。结论非特异性TCV对模型鼠的体液免疫和细胞免疫均有抑制作用。
Objective To investigate the effect of non-specific T cell vaccine (TCV) on immune function of Campylobacter jejuni-induced systemic lupus erythematosus (SLE) -like syndrome mice. Methods Formalin-treated Campylobacter jejuni CF-1 strain adjuvant-induced BALB / c mice SLE-like autoimmune syndrome pathology model, and then non-specific TCV treatment of the pathological model. Serum anti-dsDNA antibody and anti CF-OMP antibody levels were detected by enzyme-linked immunosorbent assay (ELISA), and specific delayed-type hypersensitivity (DTH) Hematoxylin-eosin (HE) staining was used to detect renal lesions. Results ① Non-specific TCV increased the level of anti-dsDNA antibody on the one hand and decreased the level of anti-CF-OMP antibody on the other hand; ② Non-specific TCV significantly inhibited the expression of Campylobacter jejuni in CF-1 model mice Sexual DTH reaction; ③ non-specific TCV can partially reduce the model of rat kidney damage. Conclusion Non-specific TCV can inhibit the humoral and cellular immunity of model mice.