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目的:观察重组人活化素A(rhAct)对百草枯所诱导的PC12细胞损伤的保护作用。方法:将百草枯、活化素A、Ldeprenyl加入体外培养的PC12细胞中,用四甲基偶氮唑盐(MTT)法检测细胞活力的变化;免疫细胞化学法和RTPCR评价细胞的酪氨酸羟化酶和Bcl2蛋白及mRNA表达水平的变化,脱氧核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡的变化,比较各组的差异。结果:预先给予活化素A和Ldeprenyl的两组细胞活力明显高于百草枯损害组,酪氨酸羟化酶和Bcl2蛋白及mRNA的表达强于损害组,同时两组的凋亡细胞明显减少,活化素A和Ldeprenyl两组间无显著差异。结论:活化素A和Ldeprenyl通过上调Bcl2的表达,抑制凋亡的发生,而对百草枯所诱导的PC12细胞损伤具有保护作用。
Objective: To observe the protective effect of recombinant human activin A (rhAct) on paraquat induced PC12 cell injury. Methods: Paraquat, activin A and Ldeprenyl were added into PC12 cells cultured in vitro. The cell viability was measured by MTT assay. Immunocytochemistry and RTPCR were used to evaluate the tyrosine The changes of the expression of Bcl2 protein and mRNA were detected by flow cytometry. The changes of apoptosis were detected by TUNEL method. The differences among groups were compared. Results: The viability of cells pre-treated with activin A and Ldeprenyl was significantly higher than that of paraquat-induced injury, and the expression of tyrosine hydroxylase and Bcl2 protein and mRNA was stronger than that of the injured group. At the same time, the apoptotic cells in both groups were significantly decreased, There was no significant difference between activin A and Ldeprenyl. CONCLUSION: Activin A and Ldeprenyl have protective effects on paraquat-induced PC12 cell injury by up-regulating the expression of Bcl2 and inhibiting apoptosis.