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目的 :研究 p5 3基因与细胞凋亡在胃癌中的检测及其之间的关系。方法 :采用 p5 3原位杂交、原位脱氧核糖核酸末端转移酶标记法 (TUNEL)和免疫组织化学技术对 5 6例胃癌进行检测。结果 :5 6例胃癌 p5 3蛋白表达 4 8.2 % ,p5 3原位杂交 6 7.9% ,二者的检测结果不完全一致 ,但均与胃癌的分期有关。晚期胃癌高于早期胃癌 ,差异有显著性 (P<0 .0 1)。 5 6例胃癌抗凋亡基因 bcl- 2阳性率为 5 8.9%。TUNEL 标记细胞凋亡指数平均为 4 .78,均与组织学分型和胃癌的分期有关 ,但又有相反的表达。未分化型腺癌和早期胃癌抗凋亡基因 bcl- 2高表达 ,而分化型腺癌和晚期胃癌低表达 ;TU NEL 显示未分化型腺癌和早期胃癌凋亡细胞指数明显高于分化型腺癌和晚期胃癌 ,差异有显著性 (P<0 .0 1)。p5 3基因与抗凋亡基因 bcl- 2呈负相关 ,与细胞凋亡指数呈正相关。结论 :胃癌 p5 3基因和细胞凋亡共同参与调节细胞的生存与死亡 ;二者均与胃癌的分期有关
Objective: To study the detection of p53 gene and apoptosis in gastric cancer and their relationship. METHODS: Fifty-six cases of gastric cancer were examined by in situ hybridization using p53, TUNEL and immunohistochemistry. Results: The expression of p53 protein in 46 cases of gastric cancer was 48.2%, and that of p53 in situ hybridization was 6.9%. The results of the two tests were not exactly the same, but all were related to the staging of gastric cancer. There was a significant difference between advanced gastric cancer and early gastric cancer (P < 0.01). The positive rate of anti-apoptosis gene bcl-2 in gastric cancer was 56.9%. The average apoptosis index of TUNEL markers was 4.78, which was related to the histological type and the stage of gastric cancer, but there was an opposite expression. In the undifferentiated adenocarcinoma and early gastric cancer, the anti-apoptotic gene bcl-2 was highly expressed, but differentiated adenocarcinoma and advanced gastric cancer were lowly expressed; TU NEL showed that the apoptotic cell index of undifferentiated adenocarcinoma and early gastric cancer was significantly higher than that of differentiated glandular There was a significant difference between cancer and advanced gastric cancer (P < 0.01). The p53 gene was negatively correlated with the anti-apoptotic gene bcl-2 and positively correlated with the apoptotic index. Conclusion: Gastric cancer p53 gene and apoptosis are involved in the regulation of cell survival and death. Both of them are related to the staging of gastric cancer.