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应用荧光光谱法研究三种原小檗碱类生物碱:盐酸小檗碱(Berberine hydrochloride)、药根碱(Jatrorrhizine)和盐酸巴马汀(Palmatine hydrochloride)与抗凋亡蛋白Bcl-2之间的相互作用,探讨了这三种生物碱对Bcl-2蛋白的荧光淬灭机理,分别测定其结合常数和结合位点数.实验结果表明,盐酸小檗碱、药根碱和盐酸巴马汀等三种原小檗碱类生物碱都能够使Bcl-2蛋白的内源性荧光发生淬灭,其荧光淬灭过程是静态淬灭过程.通过比较三种生物碱与Bcl-2蛋白的相互作用,发现随着分子柔性增强,其与Bcl-2蛋白的结合能力增强.因此,增强生物碱的分子柔性有可能增强其靶向Bcl-2蛋白的能力.
Fluorescence spectroscopy was used to study the effects of three kinds of protoberberine alkaloids: Berberine hydrochloride, Jatrorrhizine and Palmatine hydrochloride and anti-apoptotic protein Bcl-2 The mechanism of fluorescence quenching of Bcl-2 protein by these three alkaloids was discussed, and their binding constants and binding sites were determined respectively. The experimental results showed that berberine hydrochloride, jatrorrhizine and palmatine hydrochloride Protoberberine alkaloids can quench the endogenous fluorescence of Bcl-2 protein and the process of fluorescence quenching is a static quenching process.By comparing the interaction between the three alkaloids and Bcl-2 protein, It was found that as the molecule is more flexible, its binding capacity to the Bcl-2 protein is enhanced, so increasing the molecular flexibility of the alkaloid may enhance its ability to target the Bcl-2 protein.