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文内对脓毒症时血浆TNF、IL-1和IL-6水平的改变与PGE_2水平之间的关系进行了研究,同时测定了脾巨噬细胞合成的PGE_2以及布洛芬阻滞环氧化酶对脾巨噬细胞素释放的作用。选用雄性S-D鼠,重260～360g。实验前一天晚上禁食。乙醚麻醉后结扎盲肠并造成穿孔(CLP),对照组仅行剖腹探查。上述两组动物分别在术后2、5、10和20小时处死,对未经手术的大鼠行心脏穿刺采血测定血浆中细胞素和内毒素基值。结果示,实验组的血浆内毒素水平在所有4个时间点均有明显上升,其中在第2、5和20小时上升最为显著(P<0.05)。循环中TNF在头10个小时明显升高(P<0.05),高峰在2小时
The relationship between the changes of plasma TNF, IL-1 and IL-6 levels and the level of PGE 2 in sepsis was studied in this paper. The PGE 2 synthesis by splenic macrophages and the blockade epoxidation of ibuprofen Effect of Enzymes on the Release of Splenic Macrophages. Selection of male S-D rats, weight 260 ~ 360g. Fasting the night before the experiment. The cements were ligated and perforation (CLP) was induced by anesthesia in ether, while the control group was only laparotomy. The two groups of animals were sacrificed at 2, 5, 10, and 20 hours after operation respectively. Plasma concentrations of cytokines and endotoxin were determined by cardiac puncture and blood sampling in the rats without surgery. The results showed that the plasma endotoxin levels in the experimental group increased significantly at all 4 time points, with the most significant increase at the 2nd, 5th and 20th hour (P <0.05). TNF in the circulation was significantly increased in the first 10 hours (P <0.05), the peak in 2 hours