论文部分内容阅读
目的研究不同浓度葡萄糖培养环境及罗格列酮干预对分化成熟脂肪细胞血管紧张素Ⅱ受体Ⅰ(AT1R)和受体2(AT2R)基因表达的影响。方法取分化成熟的脂肪细胞3T3-L1,分别用含不同浓度葡萄糖(空白对照、5.6、11.2、25.0 mmol/L)的培养基和含不同浓度葡萄糖并添加10 nmol/L罗格列酮的培养基分组培养24 h,Real-time PCR检测3T3-L1脂肪细胞AT1R和AT2R基因表达。结果 5.6 mmol/L葡萄糖处理组AT1R基因表达明显低于空白对照和11.2、25.0 mmol/L葡萄糖处理组(P<0.05);AT2R基因表达随着葡萄糖浓度的升高而明显上调(P<0.05)。随着葡萄糖浓度的升高,罗格列酮干预组AT1R和AT2R基因表达呈下降趋势。与相应浓度的葡萄糖处理组比较,罗格列酮干预组AT1R表达均显著上调(P<0.05),作用随葡萄糖浓度的升高而减弱;AT2R基因表达显著下调(P<0.05),作用随葡萄糖浓度的升高而加强。结论罗格列酮对高浓度葡萄糖导致的脂肪细胞AT1R和AT2R基因表达变化具有调控作用。
Objective To investigate the effects of different concentrations of glucose in culture environment and the effect of rosiglitazone on gene expression of AT1R and AT2R in differentiated mature adipocytes. Methods 3T3-L1 adipocytes were differentiated and cultured in different concentrations of glucose (blank control, 5.6, 11.2 and 25.0 mmol / L) and with different concentrations of glucose and 10 nmol / L rosiglitazone Base group were cultured for 24 h. Real-time PCR was used to detect AT1R and AT2R gene expression in 3T3-L1 adipocytes. Results The AT1R gene expression in 5.6 mmol / L glucose treatment group was significantly lower than that in the blank control group and 11.2, 25.0 mmol / L glucose treatment group (P <0.05). The AT2R gene expression was significantly increased with the increase of glucose concentration (P <0.05) . With the increase of glucose concentration, the expression of AT1R and AT2R in rosiglitazone intervention group showed a decreasing trend. Compared with the corresponding glucose treatment group, the expression of AT1R in rosiglitazone intervention group was significantly increased (P <0.05), the effect was attenuated with the increase of glucose concentration; the expression of AT2R gene was significantly down-regulated (P <0.05) Concentration increased and strengthened. Conclusion Rosiglitazone regulates the expression of AT1R and AT2R genes in adipocytes induced by high concentration of glucose.