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目的探讨银杏叶提取物(EGb)对糖尿病大鼠学习记忆及海马血红素加氧酶1(HO-1)表达的影响。方法大鼠腹腔注射链脲佐菌素建立糖尿病模型,6个月后以Morris水迷宫观察其学习记忆能力,以RT-PCR观察大鼠海马HO-1 mRNA的表达,以免疫组织化学法观察海马HO-1蛋白表达。结果大鼠糖尿病6个月后Morris水迷宫成绩下降,海马的HO-1 mRNA表达和蛋白表达水平升高(分别为1．635±0．326 vs 0．978±0．214,7．2±1．7 vs 1．9±0．5,均P＜0．01)；糖尿病EGb干预组(100、50 mg/kg)大鼠较糖尿病大鼠Morris水迷宫成绩提高,HO-1 mRNA表达和蛋白表达水平降低(100 mg/kg：0．954±0．144,2．0±0．8；50 mg/kg：0．988±0．154,2．5±0．6,均P＜0．01)。结论EGb可抑制海马的HO-1表达,提高糖尿病大鼠的学习记忆能力。
Objective To investigate the effect of Ginkgo biloba extract (EGb) on learning memory and hippocampus heme oxygenase-1 (HO-1) expression in diabetic rats. Methods Diabetic models were established by intraperitoneal injection of streptozotocin in rats. Six months later, the learning and memory abilities were observed in Morris water maze. The expression of HO-1 mRNA in hippocampus was observed by RT-PCR. The hippocampus was observed by immunohistochemistry. HO-1 protein expression. Results The results of Morris water maze decreased after 6 months of diabetes in rats, and the expression of HO-1 mRNA and protein in hippocampus was increased (1.635±0.326 vs 0.978±0.214, 7.2±, respectively). 1.7 vs 1.9±0.5, all P<0.01); Diabetic EGb intervention group (100, 50 mg/kg) rats had higher Morris water maze, and the expression of HO-1 mRNA was higher than that of diabetic rats. Protein expression levels decreased (100 mg/kg: 0.954 ± 0.144, 2.0 ± 0.8; 50 mg/kg: 0.988 ± 0.154, 2.5 ± 0.6, all P < 0.01). Conclusion EGb can inhibit the expression of HO-1 in hippocampus and improve the learning and memory ability of diabetic rats.