论文部分内容阅读
采用反转录聚合酶链式反应(RT-PCR)和快速扩增c DNA末端(RACE)技术克隆桔小实蝇SOD3基因,并命名为Bdor SOD3。Bdor SOD3阅读框全长531 bp,编码176个氨基酸,第1-20位氨基酸为其信号肽区域;该蛋白序列与桔小实蝇的另外一种SOD蛋白AGE89778.1序列的一致性最高,达98.7%;采用Swiss-model在线软件模拟构建Bdor SOD3蛋白的三维结构;采用半定量PCR方法,研究Bdor SOD3基因大肠杆菌诱导后的表达情况,结果表明,Bdor SOD3在处理与对照的24 h、48 h都有表达,但Bdor SOD3在处理后48 h表达量明显升高,结果暗示Bdor SOD3与桔小实蝇蛹对大肠杆菌的免疫机制有关。
The SOD3 gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of c DNA end (RACE) technology and named as Bdor SOD3. The full-length reading frame of Bdor SOD3 was 531 bp in length and encoded 176 amino acids. The amino acids 1-20 were the signal peptide region. The sequence of Bdor SOD3 was identical with the other AGE89778.1 sequence of SOD, 98.7% respectively. The three-dimensional structure of Bdor SOD3 protein was constructed by Swiss-model online software. The semi-quantitative PCR method was used to study the expression of Bdor SOD3 gene after induced by E.coli. h, but the expression of Bdor SOD3 at 48 h after treatment was significantly increased, suggesting that Bdor SOD3 may be related to the immune mechanism of E. coli through the pupae of Trichoderma dulcis.