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目的对海洋放线菌进行分离及抗肿瘤活性筛选,并对一株具有抗肿瘤活性的海洋放线菌AH17-3的次级代谢产物进行研究。方法采用溶剂萃取、柱色谱层析及制备HPLC等方法对菌株AH17-3的发酵产物进行化学分离,通过理化性质及波谱学方法并参阅文献进行化合物结构鉴定,以SRB法评价化合物的抗肿瘤活性。结果从海洋样品中分离放线菌174株,从菌株AH17-3中分离得到了4个聚酮类化合物,经鉴定其结构分别为germicidin A(1)、germicidin B(2)、daidzein(3)、genistein(4)。其中化合物1具有弱的细胞毒活性,其IC50为3.5×10-7 M。结论海洋放线菌是重要的药用微生物资源,化合物1,2均为首次从海洋放线菌中分离得到。
Objective To isolate and screen antimicrobial activity of marine actinomycetes and to study the secondary metabolites of actinomycete marine actinomycete AH17-3 with antitumor activity. Methods The fermentation products of strain AH17-3 were isolated by solvent extraction, column chromatography and preparative HPLC. The compounds were identified by their physico-chemical properties and spectroscopic methods and their structures were identified by literature. The anti-tumor activity of compounds was evaluated by SRB . Results 174 actinomycetes were isolated from marine samples and 4 polyketides were isolated from strain AH17-3. The structures of them were identified as germicidin A (1), germicidin B (2), daidzein (3) , Genistein (4). Among them, compound 1 has a weak cytotoxic activity with an IC50 of 3.5 × 10 -7 M. Conclusion Marine Actinomycetes is an important medicinal microbial resource. Compounds 1 and 2 were isolated from marine actinomycetes for the first time.