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目的:探讨涎腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC)中神经生长因子(nerve growth factor,NGF)、神经细胞黏附分子(neural cell adhesion molecule,NCAM)、趋化因子基质细胞衍生因子-1(stromal cell derived factor 1,SDF-1;系统命名趋化因子12,CXC chemokine ligand 12,CXCL12)及其特异性受体4(CXC chemokine receptor type 4,CXCR4)表达与嗜神经侵袭的关系。方法:选取临床病理资料齐全的SACC 60例,分为嗜神经侵袭组(36例)和非嗜神经侵袭组(24例),制作组织芯片。采用免疫组织化学(immunohistochemistry,IHC)技术检测两组神经生长因子(nerve growth factor,NGF)、神经细胞黏附分子(neural cell adhesion molecule,NCAM)、CXCL12/CXCR4蛋白的表达,分析指标表达与临床病理参数的相关性。结果:NGF、CXCL12、CXCR4蛋白在SACC嗜神经侵袭组阳性表达率均高于非嗜神经侵袭组,差异具有统计学意义(P<0.05);NGF、CXCL12、CXCR4表达与患者性别、组织学类型、肿瘤发生部位及大小均无关(P>0.05)。SACC中NGF与CXCL12的阳性表达相关(r=0.279,P<0.05),也与CXCR4阳性表达相关(r=0.451,P<0.05),但CXCL12与CXCR4无明显相关(r=0.254,P=0.05)。NCAM在两组SACC中均呈阴性表达。结论:NGF、CXCL12/CXCR4过表达共同参与了SACC的嗜神经侵袭过程。
Objective: To investigate the expression of nerve growth factor (NGF), neural cell adhesion molecule (NCAM), chemokine stromal cell-derived factor (sICAM) in salivary adenoid cystic carcinoma (SACC) The relationship between stromal cell derived factor 1 (SDF-1), CXC chemokine ligand 12 (CXCL12) and its CXC chemokine receptor type 4 (CXCR4) expression and neuropathic pain . Methods: Totally 60 cases of SACC with complete clinical and pathological data were divided into neurosurgical group (36 cases) and non-neurotropic group (24 cases). The expression of nerve growth factor (NGF), neural cell adhesion molecule (NCAM) and CXCL12 / CXCR4 protein were detected by immunohistochemistry (IHC) Correlation of parameters. Results: The positive expression rate of NGF, CXCL12 and CXCR4 protein in SACC neuropathic group were significantly higher than that in non-neurotropic group (P <0.05). The expressions of NGF, CXCL12 and CXCR4 were significantly correlated with gender and histological type , Tumor location and size (P> 0.05). The positive expression of CXCL12 in SACC was correlated with the expression of CXCL12 (r = 0.279, P <0.05), but also with the expression of CXCR4 (r = 0.451, P <0.05) ). NCAM negative expression in both groups of SACC. Conclusion: Overexpression of NGF and CXCL12 / CXCR4 participate in the process of neural invasion of SACC.