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目的研究血管内皮生长因子D和血管内皮生长因子受体3(VEGF-D/VEGFR-3)在喉癌、癌周组织中的的表达情况及其在喉癌的发生和淋巴结转移中的作用,分析其与喉癌的临床病理参数的关系及相关性。方法选择46例喉癌患者的手术切除标本、14例癌周组织标本,采用免疫组化SP法,观察VEGF-D/VEGFR-3在喉癌中表达的相关性及与患者的年龄、性别、临床TNM分期、病理类型、肿瘤的原发部位和淋巴结转移的关系。结果①喉癌组织中VEGF-D/VEGFR-3表达的阳性率明显高于癌周组织,两组间比较差异有统计学意义(P<0.01)。②VEGF-D/VEGFR-3阳性表达率与肿瘤的分化程度、淋巴结转移和临床TNM分期密切相关(P<0.05),而与年龄、肿瘤原发部位及性别无关(P>0.05)。结论 VEGF-D可作为喉癌预后的一种新指标,阻断VEGF-D/VEGFR-3通路将有望成为喉癌淋巴结转移预防和治疗的新靶点。
Objective To investigate the expression of vascular endothelial growth factor D and vascular endothelial growth factor receptor 3 (VEGF-D / VEGFR-3) in laryngeal cancer and peri-cancerous tissues and their roles in the carcinogenesis and lymph node metastasis of laryngeal carcinoma, Analyze the relationship between the clinicopathological parameters of laryngeal cancer and its correlation. Methods Forty-six patients with laryngeal cancer were selected for surgical resection and 14 cases of periocular tissue. Immunohistochemical SP method was used to observe the correlation between the expression of VEGF-D / VEGFR-3 and the age, sex, Clinical TNM staging, pathological types, the primary site of the tumor and lymph node metastasis. Results ① The positive rate of VEGF-D / VEGFR-3 expression in laryngeal carcinoma was significantly higher than that in peri-cancerous tissues, the difference was statistically significant (P <0.01). ② The positive rate of VEGF-D / VEGFR-3 was closely related to the degree of differentiation, lymph node metastasis and TNM staging (P <0.05), but not to the age, tumor location and sex (P> 0.05). Conclusion VEGF-D can be used as a new prognostic marker for laryngeal cancer. Blocking VEGF-D / VEGFR-3 pathway may be a new target for the prevention and treatment of lymph node metastasis in laryngeal cancer.