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目的 观察急性躯体 心理应激对大鼠心血管活动的影响及心血管相关脑区c fos蛋白的表达。 方法 采用足底电击结合噪声的应激模型,检测应激后SD大鼠血压、心率和脑内c fos蛋白表达等变化。 结果 (1)应激即刻引起血压和心率明显增加。收缩压从应激前(10 4 .8±9.4 )mmHg升至(132 .3±10 .7)mmHg ,明显高于对照组[(112 .0±8.0 )mmHg ,(P <0 .0 1) ];应激大鼠心率明显高于应激前和对照组(P <0 .0 1)。应激停止后2～3h ,血压和心率恢复到对照水平。(2 )应激组大鼠杏仁核群c fos蛋白阳性细胞为(2 5 .4±3.8) % ,明显高于对照组[(3.4 1±0 .5 ) % ,(P <0 .0 1) ]。下丘脑室旁核c fos蛋白阳性细胞为(2 1.9±2 .0 ) % ,与对照组(7.1±0 .8) %比较,差异有显著性(P <0 .0 5 ,n =8)。额颞叶皮层c fos蛋白阳性细胞为(5 7.5±3.9) % ,显著高于对照组[(16 .4±2 .5 ) % ,(P <0 .0 1,n =8) ]。 结论 急性躯体 心理应激引起的快速心血管反应可能与下丘脑室旁核、杏仁核群神经元激活有关。
Objective To observe the effects of acute somatic psychological stress on cardiovascular activity and c fos protein expression in cardiovascular related brain regions. Methods The stress model of foot shock combined with noise was used to detect the changes of blood pressure, heart rate and c fos protein expression in SD rats after stress. Results (1) Immediate stress caused by blood pressure and heart rate increased significantly. Systolic blood pressure increased from (104.4 ± 9.4) mmHg to (132.3 ± 10.7) mmHg before the stress, which was significantly higher than that of the control group [(112.0 ± 8.0) mmHg, P <0.01 )]. The heart rate of stressed rats was significantly higher than that before stress and control (P <0.01). 2 to 3 hours after the cessation of stress, blood pressure and heart rate returned to the control level. (2) The positive rate of c fos protein in amygdala group in stress group was (2.54 ± 3.8)%, which was significantly higher than that in control group [(3.4 1 ± 0.5)%, P <0.01 )]. The positive rate of c fos protein in paraventricular nucleus of hypothalamus was (2.19 ± 2.0)%, which was significantly different from that of control group (7.1 ± 0.8)% (P <0.05, n = 8) . The number of c fos protein positive cells in the frontotemporal cortex was (7.5 ± 3.9)%, which was significantly higher than that in the control group (16.4 ± 2.5%) (P <0.01, n = 8). Conclusion The rapid cardiovascular response induced by acute physical stress may be related to the activation of neurons in paraventricular nucleus and amygdala of hypothalamus.