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[Objective] To study the optimal extraction technical parameters of flavonoids glycosides from HERBA LOBELIAE CHINENSIS by ultrasonic-assisted extraction.[Method] With ethanol as the extracting solvent,the orthogonal design was employed to test the effects of four factors like ethanol concentration,extraction time,extraction temperature and solid-liquid ratio.[Result] The optimum conditions for extracting flavonoids glycosides from HERBA LOBELIAE CHINENSIS were as follows:ethanol concentration of 50%,extraction time of 35 min,extraction temperature of 50 ℃ and solid-liquid ratio of 1:50.The content of falconoid glycosides could reach 4.209 2 mg/g under the optimal conditions.[Conclusion] High in extraction rate and good in stability,the method favored the determination of flavonoids glycosides from HERBA LOBELIAE CHINENSIS.
[Objective] To study the optimal extraction technical parameters of flavonoids glycosides from HERBA LOBELIAE CHINENSIS by ultrasonic-assisted extraction. [Method] With ethanol as the extracting solvent, the orthogonal design was employed to test the effects of four factors like ethanol concentration, extraction time, extraction temperature and solid-liquid ratio. [Result] The optimum conditions for extracting flavonoids glycosides from HERBA LOBELIAE CHINENSIS were as follows: ethanol concentration of 50%, extraction time of 35 min, extraction temperature of 50 ° C and solid-liquid ratio of 1: 50. The content of falconoid glycosides could reach 4.209 2 mg / g under the optimal conditions. [Conclusion] High in extraction rate and good in stability, the method favored the determination of flavonoids glycosides from HERBA LOBELIAE CHINENSIS.