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目的探讨重组人改构体酸性成纤维细胞生长因子(Mrh-aFGF)对帕金森病(PD)大鼠黑质神经元病变的影响。方法 SD大鼠72只,随机分为4组:对照组、PD组、生理盐水(NS)处理组、Mrh-aFGF处理组,每组18只。6-OHDA分别注入左侧黑质和腹侧被盖区后建立PD大鼠模型,侧脑室内注射Mrh-aFGF,用阿扑吗啡诱导旋转行为;Nissl染色法观察大鼠黑质神经元病理学改变;电子显微镜观察黑质神经元超微结构的变化。结果对照组均未出现旋转行为;PD组术后旋转速度逐渐加快;NS处理组旋转行为未见明显改善;Mrh-aFGF处理组旋转速度明显减慢(P<0.05);PD组及NS处理组大鼠损毁侧黑质神经元数目较健侧显著减少(P<0.05);Mrh-aFGF处理组术后1周、2周、4周损毁侧神经元数目均较PD组及NS处理组明显增加(P<0.05);PD组大鼠黑质神经元超微结构明显受损,出现核固缩,线粒体肿胀、嵴消失,粗面内质网扩张、脱颗粒,以及突触前后膜肿胀,突触间隙消失,Mrh-aFGF处理组黑质神经元超微结构有明显改善。结论 Mrh-aFGF能改善PD大鼠的旋转行为,减少PD大鼠黑质神经元的丢失,并改善其黑质神经元的超微结构。
Objective To investigate the effect of recombinant human recombinant human fibroblast growth factor (Mrh-aFGF) on the changes of substantia nigra neurons in Parkinson’s disease (PD) rats. Methods Seventy-two SD rats were randomly divided into 4 groups: control group, PD group, NS group and Mrh-aFGF group, with 18 rats in each group. 6-OHDA were injected into the left substantia nigra and ventral tegmental area to establish PD model rats, intracerebroventricular injection of Mrh-aFGF, apomorphine induced rotation behavior; Nissl staining of rat substantia nigra neuronal pathology Changes; ultrastructure of substantia nigra neurons observed by electron microscopy. Results Rotation speed of rats in PD group was faster than that in control group. No significant improvement of rotation was observed in NS group. Rotation speed of Mrh-aFGF group was significantly decreased (P <0.05). PD group and NS group The number of injured neurons in injured side of rats significantly decreased compared with that of uninjured side (P <0.05). The numbers of injured neurons in injured group of Mrh-aFGF at 1 week, 2 weeks and 4 weeks after operation were significantly higher than those of PD and NS (P <0.05). The ultrastructure of substantia nigra neurons in PD group was significantly impaired, nuclear pyknosis, swelling of mitochondria, disappearance of ridges, dilation of rough endoplasmic reticulum, degranulation and swelling of the anterior and posterior synapses Touch the gap disappeared, Mrh-aFGF treatment group significantly improved ultrastructure of substantia nigra neurons. Conclusion Mrh-aFGF can improve the rotation behavior of PD rats, reduce the loss of substantia nigra neurons and improve the ultrastructure of substantia nigra neurons in PD rats.