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本文通过对18例慢性扁桃腺炎等淋巴网状组织的新鲜标本立即作印片与按Mueller氏法处理行甲醇致冷器冰切与石腊切片作ANAE染色。并将剩余组织用10％甲醛液固定,然后在第10、20、30天分别取组织块作甲醇致冷器冰切与石腊切片作ANAE染色,检测各种标本的AN AE的点状阳性细胞率,相互作比较分析。其结论是新鲜组织甲醇致冷器冰切的阳性率最高,其次为印片与石腊切片。甲醛固定组织则存放时间愈长,ANAE酶活性愈低,直到第30天者全部消失。
In this paper, 18 specimens of chronic tonsillitis and other lymphoid reticular tissues were immediately printed and processed by the Mueller’s method. Methanol cooler cryosection and paraffin sections were used for ANAE staining. The remaining tissues were fixed with 10% formaldehyde, and then the tissue blocks were taken on the 10th, 20th, and 30th day respectively for ice-cutting with a methanol cooler and paraffin sections for ANAE staining. Dot-like positive AN AE was detected on various specimens. Cell rates, comparative analysis of each other. The conclusion is that the positive rate of ice cutting by fresh tissue methanol cooler is the highest, followed by printed and paraffin sections. The longer the formaldehyde-fixed tissue was stored, the lower the ANAE enzyme activity was, and all disappeared by the 30th day.