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目的探讨电场暴露对晶状体上皮伤口愈合的作用。设计实验研究。研究对象原代培养的牛晶状体上皮细胞。方法钝头玻璃针戳刺原代培养的牛晶状体上皮细胞单层形成圆形微伤口,暴露于场强为200mV/mm的直流电场2h(n=37),未受电场暴露的伤口作为对照(n=29),由Leica图像分析系统测量伤后0h、1h和2h的伤口面积。用抗F-actin荧光抗体染色观察伤口细胞微丝骨架。主要指标伤口面积及细胞微丝骨架的排列。结果与对照伤口比较,电场暴露伤口愈合延迟,电场暴露伤口伤后2h的平均面积(19106μm2±2167μm2)明显大于未接受电场暴露的对照伤口(8555μm2±1911μm2)(t=2.942,P=0.0045)。对照伤口细胞微丝骨架呈向心性排列,电场暴露伤口细胞微丝骨架则沿伤缘呈“索状”排列。结论生理强度的外源性电场可抑制晶状体上皮伤口的愈合。
Objective To investigate the effect of electric field exposure on the healing of lens epithelial wounds. Design experiment research. Study the primary cultured bovine lens epithelial cells. Methods Monolayers of primary cultured bovine lens epithelial cells were punctured with blunt glass needles to form circular micro-wounds exposed to DC electric field of 200 mV / mm for 2 h (n = 37), and those not exposed to electric field were used as controls n = 29). The wound area at 0 h, 1 h, and 2 h after injury was measured by a Leica image analysis system. The fibroblast scaffolds were stained with anti-F-actin fluorescent antibody. The main indicators of wound area and cell microfilament skeleton arrangement. Results Compared with the control wounds, wound healing after electric field exposure was delayed. The average area at 2h after electric field exposure (19106μm2 ± 2167μm2) was significantly larger than that of control wounds without exposure to electric field (8555μm2 ± 1911μm2) (t = 2.942, P = 0.0045). Wound microflora of control cells showed centripetal arrangement, and the microfilament scaffolds exposed by electric field showed a “cord-like” arrangement along the wound edge. Conclusion Physiological strength of the exogenous electric field can inhibit the healing of lens epithelial wounds.