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本文以兔网织红细胞为材料,制备溶胞液,建立了体外无细胞蛋白质翻译系统,从氨基酸参入量反映其有较高的翻译活力,该系统可进一步适用于对外源mRNA的翻译研究。
In this paper, rabbit reticulocytes were used as materials to prepare lysis solution, and to establish a cell-free protein translation system in vitro. The amino acid incorporation reflected the high translational activity. This system can be further applied to the translation studies of exogenous mRNAs.