建立了小鼠脾脏、胸腺细胞体外转化微量培养系统,确定了最佳实验条件.点蜡法充CO_2,简便易行,效果好.RPMI-1640培养基内补充胎牛血清,其浓度确定为在脾细胞培养为10%,在胸腺细胞培养为20%.脾细胞培养最适细胞密度为2～5×10~6/ml,Con A最适量为2～4μg/培养;胸腺细胞培养细胞最适密度为1×10~7/ml,Con A最适量为2～4μg/培养.两种已知免疫活性的药物在脾细胞培养体系有明确肯定的反应. The mouse spleen and thymus cells were transformed into micro-culture system in vitro, and the best experimental conditions were determined.It was easy and feasible to fill CO_2 with wax method.The fetal bovine serum was supplemented in RPMI-1640 medium and its concentration was determined as The spleen cell culture was 10% and thymocyte culture was 20% .The optimal cell density of spleen cell culture was 2 ~ 5 × 10 ~ 6 / ml, the optimal amount of Con A was 2 ~ 4μg / The density of 1 × 10 ~ 7 / ml, Con A optimum amount of 2 ~ 4μg / culture. Two known immunocompetent drugs in the spleen cell culture system has a clear and positive response.