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目的 研究壳聚糖对低密度脂蛋白和脂蛋白 (a)代谢的影响。方法 壳聚糖经剌猬腋下静脉注入 ,2min后给予12 5I标记低密度脂蛋白、12 5I标记脂蛋白 (a)、12 5I标记去唾液酸低密度脂蛋白或12 5I标记去唾液酸脂蛋白 (a) ,1h后处死动物 ,测定血、肝、肾、脾、胆汁和肾上腺中的放射性含量。结果 实验发现肝对去唾液酸低密度脂蛋白和去唾液酸脂蛋白 (a)摄入均增加 ,但去唾液酸低密度脂蛋白被肝重新利用释放入血 ,而去唾液酸脂蛋白 (a)被大部分排出体外。由于肾和肾上腺亦能增加对去唾液酸低密度脂蛋白的摄取 ,因此不排除低密度脂蛋白受体参与去唾液酸低密度脂蛋白代谢 ,但去唾液酸脂蛋白 (a)和一部分去唾液酸低密度脂蛋白可能经去唾液酸脂蛋白相关受体代谢。壳聚糖能使肝和肾上腺对低密度脂蛋白的摄入增加 ,同时使血中低密度脂蛋白浓度降低 ,脾细胞对低密度脂蛋白的摄取也明显降低。结论 实验提示壳聚糖可能通过激活低密度脂蛋白受体起到降脂作用。
Objective To study the effects of chitosan on the metabolism of low density lipoprotein and lipoprotein (a). Methods Chitosan was infused into the armpit vein of hedgehog. After 2 minutes, 125 I labeled low density lipoprotein, 125 I labeled lipoprotein (a), 125 I labeled asialoglycoprotein (LDL) or 125 I labeled asialool (A) After 1 h, animals were sacrificed to determine the radioactive content in blood, liver, kidney, spleen, bile and adrenal glands. Results The liver was found to have increased uptake of asialoglycoprotein and asialoprotein (a), but desalinated low density lipoprotein was released into the blood by the liver, whereas aspartate lipoprotein (a Mostly excreted. As the kidneys and adrenal glands also increase uptake of asialo low density lipoprotein, it is not excluded that low density lipoprotein receptor is involved in asialo low density lipoprotein metabolism, but asialoprotein (a) and part of desalin Acid low density lipoproteins may be metabolized to asialo-lipoprotein-associated receptors. Chitosan can increase the liver and adrenal intake of low-density lipoprotein, while reducing the concentration of blood low-density lipoprotein, spleen cells uptake of low-density lipoprotein was also significantly reduced. Conclusion The experimental results suggest that chitosan may reduce lipid levels by activating low density lipoprotein receptor.