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目的探讨乙型肝炎表面抗原(HBsAg)联合重组鼠白介素-12(rmIL-12)对免疫小鼠诱导细胞免疫应答的影响。方法以不同给药方式、不同剂量的HBsAg联合rmIL-12免疫C57BL/6J小鼠,ELISA法检测小鼠血清及脾淋巴细胞中IFNγ及IL-4的水平。结果HBsAg+rmIL-12高剂量组免疫的C57BL/6J小鼠,其脾淋巴细胞IFNγ水平明显高于BALB/c小鼠;3种不同给药方式均可诱导C57BL/6J小鼠血清和脾细胞IFNγ水平明显升高,三者之间差异无统计学意义;HBsAg+rmIL-12高、中、低剂量组免疫C57BL/6J小鼠血清可诱导IFNγ水平明显升高,且呈剂量依赖性,脾细胞IFNγ水平在中、低剂量组均未产生效应,高剂量组明显升高,而对IL-4无明显影响。结论rmIL-12可显著增强HBsAg诱导的细胞免疫应答,并使免疫应答转向Th1型,对于发展治疗性乙肝疫苗具有潜在的应用价值。
Objective To investigate the effect of hepatitis B surface antigen (HBsAg) combined with recombinant murine interleukin-12 (rmIL-12) on the induction of cellular immune response in immunized mice. Methods C57BL / 6J mice were immunized with different doses of HBsAg and rmIL-12 at different doses. The levels of IFNγ and IL-4 in serum and splenic lymphocytes were detected by ELISA. Results C57BL / 6J mice immunized with HBsAg + rmIL-12 high dose group had significantly higher IFNγ levels of splenic lymphocytes than those of BALB / c mice. C57BL / 6J mice immunized with HBsAg + rmIL- IFNγ levels were significantly increased, the difference between the three was not statistically significant; HBsAg + rmIL-12 high, medium and low doses of serum immunized C57BL / 6J mice induced IFNγ levels were significantly increased, and in a dose-dependent manner, spleen Cytokine IFNγ levels in the medium and low dose groups did not produce any effect, high dose group was significantly increased, but no significant effect on IL-4. Conclusion rmIL-12 can significantly enhance the HBsAg-induced cellular immune response and shift the immune response to Th1 type, which has potential value for the development of therapeutic hepatitis B vaccine.