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目前盐溶蛋白电泳是种子企业鉴定玉米种子纯度的最主要方法之一。玉米种子盐溶蛋白(属于贮藏蛋白)分子量分布范围较窄,但蛋白质组分之间的电荷差别较大,通过电泳技术的浓缩效应、分子筛效应、电荷效应的作用下,可以把蛋白质区分开,经过染色显示蛋白质谱带类型。蛋白质的不同是由内部的DNA决定的,利用蛋白质的谱带差异来鉴定品种的真实性和品种纯度。2001年,已作为国家农业行业标准(NY/T449-2001)。在种子企业内部,提高盐溶蛋白电泳在玉米种子纯度鉴定中的应用水平迫在眉睫。1建立标准图谱
At present, salt-soluble protein electrophoresis is one of the most important methods for seed enterprises to identify the purity of corn seeds. The molecular weight distribution of the maize seed salted protein (belonging to the storage protein) is narrow but the difference of the charges between the protein components is large. The protein can be distinguished by the effect of concentration, molecular sieve effect and charge effect of the electrophoresis technique, Stained to show the type of protein band. The difference between proteins is determined by the internal DNA, the use of protein bands to identify differences in the authenticity of varieties and varieties of purity. 2001, has been as a national agricultural industry standards (NY / T449-2001). It is imminent to improve the application of salt-soluble protein electrophoresis in the identification of maize seed purity within seed enterprises. 1 Create a standard map