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采用高静水压法诱变了黄瓜品种S9925-3,产生了两个稳定的可遗传变异株。采用30对随机配对引物,对该品种的原种及两个变异株进行了扩增片段长度多态性(AFLP)分析。结果表明原种与两个变异植株之间存在明显的多态性。从聚丙烯酰胺凝胶上切下6个差异明显的条带进行DNA测序,并将获得的DNA序列与NCBI(National Center for Biotechnology Information)数据库中的黄瓜DNA数据进行比对分析,确定了变异位点,找到了变异黄瓜系表型变化的可能原因。首次完成了对高静水压处理引起的黄瓜稳定遗传变异系的分子水平分析。研究结果表明,高静水压技术是一种有效的改良作物品系的新诱变工具。
Cucumber variety S9925-3 was mutagenized by high hydrostatic pressure and two stable heritable variants were produced. Thirty pairs of random primers were used to analyze the AFLP of the original and two mutant strains. The results showed that there was a significant polymorphism between the original species and two mutant plants. Six distinct bands were excised from polyacrylamide gel for DNA sequencing and the obtained DNA sequences were aligned with cucumber DNA data from the NCBI database to determine the number of variants Point to find the possible causes of phenotypic changes in cucumber lines. The molecular level analysis of the stable genetic variation of cucumber caused by high hydrostatic pressure was completed for the first time. The results show that high hydrostatic pressure technology is an effective tool to improve the strain of the new mutagenesis.